Among all the vaccine adjuvants, which are present in the market, it is the Mouse Adjuvant that is said to be quite an efficient one that can be used for every kind of immnogen that is tested. The criteria for the same, is rapidness to raise the immune responses along with antibody tiers, which are produced. It is also extremely safe as it is regarded to be a non toxic adjuvant, and does not come with any side effects for the animals.Magic mouse adjuvant stands out from the rest not only because it’s the best in quality but also because it is, adjuvant sparing and immunogen. This effectively means that very less immunogen would be needed than the conventional procedures that are being used. Also it is extremely time-saving. This is due the fact that it is possible to derive within 35 days, very strong immune responses, after immunization. It is also highly valuable as it produces more antibodies against the conformational epitopes.
After receiving the adjuvant, the same is required to be stored at around 2-80C. This adjuvant stays stable for a period of 1 year, however, needs to be stored at 2-80C. There are two divisions in the protocol, namely boosting and priming. The latter is needed for regain the activated B-Cells for creating hybridomas for production of monoclonal antibodies.
Then it becomes necessary to calculate the total immunogen amount, have it diluted with any animal or PBS and then compatible buffer to around two folds of the final concentration in the adjuvant-immunogen mixture. Next, the immunogen is to be mixed with Adjuvant in the ratio of 1:1 and have it pippeted up gently down and up to around five times. Now, inject adjuvant/immunogen mix of about 100 µL and have it mixed into the quadriceps muscle of every mouse. One can view the precipitation present within the adjuvant/immunogen or adjuvant mix. It needs to be mixed well before being drawn into a syringe to be injected quickly after it is drawn into the syringe. By following the above steps of the priming protocol above the animals can be boosted for about three weeks right from the first immunization. It is said that serum titer tends to peak about thirty five days after the prime immunization. Then from the tail tips, bleed after two weeks following the initial boost and measure through ELISA, the antibody titers.
The mentioned protocol tends to describe boosting for three weeks right after the prime (initial) immunization.
But, boosting could also be conducted eight weeks after initial immunization. Glyphosate testing is fast gaining popularity these days in the field of agriculture. Glyphosate (N-(phosphonomethyl glycine) is a widely known broad-spectrum systemic herbicide. Glyphosate testing is used to kill the weeds, especially the broadleaf weeds and grasses which pose a threat to the commercial crops grown in and around the world. According to company sources, they have been doing their best to produce high quality vaccinations of different types.
Contact Info ::
45-16 Ramsey Road, Shirley, NY 11967, USA
Phone: 1-631-624-4882
Fax: 1-631-614-7828
Email: info@creative-diagnostics.com
visit US : http://www.creative-diagnostics.com